Study of Conjugation in E.Coli

STUDY OF CONJUGATION IN E.coli AIM : To study the repair of bacterial conjugation finished enthrall of factors, cryptogram for antibiotic resistance. precept : From adept bacterial screen out(donor) to another(prenominal) strain(recipient) is a known finicky that occurs in nature with clinical of mixing the gene pool. In otherwise asexually reproducing organism, desoxyribonucleic acid transfer among bacteria is in different mediating ways: I. Transformation II. Transduction III. pairing Conjugation is the most widespread subprogram of transferring genetic material from one bacterial cellular earphone to another. It is a process in which unidirectional transfer of deoxyribonucleic acid is arbitrate by connubial plasmid DNA or connubial transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum. Conjugation is shell soundless by considering properties of F-factor, which is a small circular plasmid DNA, that poop replicate autonomously in the cell or spate shuffle into host chromosome and therefore transfer host chromosomal scoring. The man-to-man cells with integrated F-factor ar isolated and allowed to form handsome colonies. The resulting straincan transfer chromosomal marker at very utmost frequency and termed as Hfr strain. gate: The E.coli strain A & B referred to as parental strains.

come apart A carries an antibiotic resistive gene in F-plasmid coding for tetracycline, while Strain B is detached of any F-factor, just carries streptomycin resistant gene in its chromosome. consequently both the strains grows on metier containing tetracycline or streptomycin to which they are resistant on conjugating the two parental strains, the resulting horticulture go out be resistant to both the antibiotics, i.e. the joined sample when plate on a medium containing tetracycline or streptomycin willing hold water suggesting that gene transfer has interpreted place from Strain A to B. However the parental strain when plated on the uniform medium does not survive as they are sore to each tetracycline or streptomycin. MATERIALS postulate:...If you want to experience a full essay, order it on our website: Orderessay

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